It is advantageous for treating Hypertension i.e. 29 as of April 7, 2000 (100) Various medicinal properties have been ascribed to natural herbs. These results strongly suggest that SHWE enhances heat stress tolerance in C. elegans through activation of HSP-16.2 expression. Antioxidant properties of Sideritis may prevent from Cancer. This plant has so many advantages I hardly know where to begin: Soft, velvety, bluish-white foliage with odd curving flower spikes of soft yellow that attract all manner of small pollinators — a beautiful perennial white-foliage plant Individuate seedlings to gallon pots and grow until sufficiently sized to transplant successfully to garden or landscape. Sunagawa, T., Shimizu, T., Kanda, T., Tagashira, M., Sami, M., Shirasawa, T. Prasanth, M., Sivamaruthi, B., Chaiyasut, C., Tencomnao, T. Guerrero-Rubio, MA., Hernández-García, S., García-Carmona, F., Gandía-Herrero, F. View or download all content the institution has subscribed to. *P < 0.05, compared with control. Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. CONCENTAL is made from the extracts of selected Sideritis species, commonly called Greek Mountain Tea, which have been famous for their healing powers throughout history. The antiaging effects of SHWE revealed in this study may be due partially to the phenolic compounds contained. If you have access to a journal via a society or association membership, please browse to your society journal, select an article to view, and follow the instructions in this box. These plants will have a lot more details displayed including an image. The plant Pirin tea (Sideritis scardica Grsb.) If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Among the tested groups, the maximum life span of N2 worms treated with 50 µg/mL SHWE was the longest, at 25 days. Each TJ375 worm L1 larvae was placed on the mNGM agar plate supplemented with SHWE to give final concentrations of 100, 500, and 1000 µg/mL, and preincubated for 72 hours at 20°C. Flowers make a pleasant, relaxing tea HSP-16.2 is a small HSP in C. elegans, and its expression is induced by heat and oxidative stress.15 It has been pointed out that increasing the expression level of HSP-16.2 in cells has a prolonged life span effect on C. elegans.16 The TJ375 strain of C. elegans is a genetically modified worm with a hsp-16.2 promoter linked to a green fluorescent protein (GFP) reporter, enabling visualization of HSP-16.2 expression in response to the stress. The data are expressed as mean ± standard error. As a result of thin-layer chromatography analysis using the Folin-Ciocalteu reagent as a spray reagent, several spots suggesting phenolic compounds were detected in SHWE (data not shown). Sideritis scardica (known as Ironwort, “Olympus tea”, “Pirin Tea”, or “Mursalski Tea”) is traditionally used in the Balkans and the Middle East for the preparation of herbal tea for strengthening For the life span assay, the statistical significance was determined by a log-rank test. Figure 2 Effect of hot water extract of Sideritis scardica (SHWE) on heat shock protein-16.2::green fluorescent protein expression in TJ375 worm. The experiment was performed with 50 worms of type N2 and 70 worms of type GR1307. They are abundant in Mediterranean regions, the Balkans, the Iberian Peninsula and Macarones DAF-16 is the only homolog of the FOXO family of transcription factors in C. elegans and has been shown to be involved in the expression of HSP-16.2.17,18 Normally, DAF-16 is phosphorylated by DAF-2 and AKT-1/AKT-2 in the insulin/insulin-like growth factor-1 signaling pathway and is present in the cytoplasm.19 Under stress conditions such as heat shock, peroxidation, and starvation, phosphorylation of DAF-16 is suppressed, and as a result, dephosphorylated DAF-16 translocates to the nucleus and induces the expression of stress-responsive genes such as hsp-16.2 and sod-3.18,20 To determine whether DAF-16 is involved in the activation of HSP-16.2 expression induced by SHWE treatment, changes in the subcellular localization of DAF-16 were analyzed using TJ356 worms, genetically modified strain of C. elegans. Worms were sorted and counted according to observed amounts of localized DAF-16::GFP. We thank Dr Wataru Aoki of the Graduate School of Agriculture, Kyoto University for advice on bioassays using C. elegans. It has been reported that plant extracts such as blueberry, apple, and green tea show life-extending effects and stress-relieving effects in experiments using C. elegans.23-25 In these extracts, polyphenols (proanthocyanidins) are contained as a main component, and it has been pointed out that they contribute to the antiaging effects.23-25 In another study,10 it has been reported that polyphenols are contained in the water-soluble extract of S. scardica. As a result of the test, the worms pretreated with SHWE significantly maintained a higher survival rate than the control group (Figure 5). This listing is for Greek Mountain Tea starter seedling. Ultrapure water sterilized by filtration was used as a control solution. Woody perennial growing to 18 inches, native to the mountains surrounding the Mediterranean sea, especially the Peloponnese. Simply select your manager software from the list below and click on download. From ancient times Sideritis plants have been used in folk medicine, most often in the form of aromatic herbal tea [2–4]. Tea of dried flowering tops is sweetish and pleasant. Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. The tea of S. scardica has been hand - ed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. *P < 0.01, compared with control. After incubation, each strain of worm was transferred to a sample-free NGM plate and exposed for 8 hours to 35°C. The tea of S. scardica has been handed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. Age-synchronization of the worms was performed according to the method of Guerrero-Rubio et al.26. I water it once a month or so from June to September and don’t fertilize it at all now that the plants are fully established. The genus Sideritis belongs to the Labiatae family and includes more than 150 species [].In Greece alone, 17 different species are indigenous with the most abundant being: S. perfoliata sub athoa, S. clandestina, S. scardica, S. raeseri, S. syriaca and S. euboea. Sideritis syriaca - is an albanian mountain tea. Sideritis scardica & raiseri Sideritis (also known as ironwort, mountain tea and shepherd's tea) is a genus of flowering plants well known to man for their medicinal properties. Antioxidant activity equal to green tea. The life span of wild-type N2 worms was prolonged by approximately 15% at the SHWE concentration of 5 µg/mL and approximately 22% at the SHWE concentration of 50 µg/mL, as compared with the control group. As a second step, we analyzed the expression of HSP and DAF-16/FOXO using transgenic mutants of C. elegans and then investigated the bioactivities of S. scardica. As an herbal tea (herbal infusion technically) it is known for its antioxidants, which come in the form of polyphenols and flavonoids. Figure 1 shows the survival curves of N2 worms cultured on modified nematode growth medium (mNGM) agar plates containing SHWE under normal culture conditions. Aging is a progressive and complex process that impairs vital and repair functions and causes aging-related disorders such as cardiovascular and neurodegenerative diseases and cancer.1 Suppression of aging and aging-related degeneration would greatly contribute to improving the quality of life of humans. The dried plant of Bulgarian S. scardica was kindly donated by Koshin bussan. After that, randomly selected worms were transferred to sample-free NGM agar plates and exposed to heat stress for 2 hours at 35°C. Blooms all year in zone 9, and very heavily in late spring and summer. Sideritis Plant Sideritis is a genus of flowering plants that typically grow in the Mediterranean countries. Your Shopping Cart will be saved with Product pictures and information, and Cart Totals. The results were obtained from 7 independent experiments with 10 worms in each experimental group. The data are expressed as mean ± standard error. Introduction. Furthermore, a survival test under heat stress was performed using GR1307 worms, genetically modified strain in which the daf-16 gene region was knocked out. For those with a naturalist bent it’s a fascinating laboratory but in any case providing a sheltered spot for spiders in the garden is always a good idea. A password will be sent to your email address. Lang, IA., Scarlett, A., Guralnik, JM., Depledge, MH., Melzer, D., Galloway, TS. High Blood Pressure. By continuing to browse It keeps a check on the growth of Cancerous Tumor. Rated 5.00 out of 5 based on 2 customer ratings (4 customer reviews) $ 7.50. The mammalian genome encodes 4 FOXO proteins, while genomes of Caenorhabditis elegans and Drosophila melanogaster encode only 1 FOXO homolog, DAF-16 and dFOXO, respectively.7 FOXO is responsible for activating genes involved in various cellular stress responses including autophagy, apoptosis, and stress resistance.8 Therefore, it has been pointed out that increasing cellular expression of these proteins may contribute to prolonging the life span of living organisms. Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. Traditional usage (TWM): colds and restless insomnia. No change in the subcellular localization of DAF-16 was observed in all groups pretreated with SHWE under heat stress. L1 worms preincubated with SHWE for 72 hours at 20°C were exposed to heat stress for 8 hours at 35°C on the nematode growth medium agar plates. Albanian people have been using it as a tonic for many years. Hardy to Zones 7 to 10. http://www.ncbi.nlm.nih.gov/pubmed/22975005, http://www.ncbi.nlm.nih.gov/pubmed/9562038, Autophagy following heat stress: the role of aging and protein nitration, http://www.ncbi.nlm.nih.gov/pubmed/18758235, Age-related impairments of mobility associated with cobalt and other heavy metals: data from NHANES 1999-2004, http://www.ncbi.nlm.nih.gov/pubmed/19199147, Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance, http://www.ncbi.nlm.nih.gov/pubmed/11960972, Role of FOXO transcription factors in crosstalk between mitochondria and the nucleus, http://www.ncbi.nlm.nih.gov/pubmed/28417222, Foxo transcription factors in the maintenance of cellular homeostasis during aging, http://www.ncbi.nlm.nih.gov/pubmed/18394876, http://www.ncbi.nlm.nih.gov/pubmed/24487281, Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. *P < 0.05, **P < 0.01, compared with control. 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